Publications by authors named "Asmatullah"

11 Publications

  • Page 1 of 1

Evaluation of sex steroid hormones and reproductive irregularities in diethyl phthalate-exposed premature mice: modulatory effect of raw honey against potential anomalies.

Environ Sci Pollut Res Int 2021 Jun 15. Epub 2021 Jun 15.

Institute of Zoology, University of the Punjab, Lahore, Pakistan.

Phthalates, plasticizing chemicals, are top-rated environmental contaminants. Diethyl phthalate (DEP), a chief member of this family, was declared a potent endocrine disruptor and carcinogen in animals and humans. The current study was designed to explore the probable reproductive damage induced by DEP and the therapeutic efficacy of raw honey in male albino mice. Four-week-old 50 male mice were randomized equally in five groups, as control (C) received 0.1 ml distilled water; vehicle control (VC) received corn oil (0.1 ml/mouse); DEP (3mg/g/BW) dissolved in corn oil; honey control (HC) administered with honey (0.2 mg/g/day); and phthalate plus honey (P+H) administered with DEP and honey (3mg and 0.2 mg/g/BW/day respectively). Mice were treated through oral gavage for 54 days routinely, acclimatized for 6 days, and dissected. In the first instance, the antioxidant potential and total phenolic contents (TPC) of honey were analyzed through ferric reducing antioxidant power (FRAP) assay and Folin-Ciocalteu assay to confirm the antioxidant capacity of honey. The morphological, morphometric, histological, micrometric, sperm count, and hormonal analyses, and antioxidant capacity test in tissue homogenates were conducted by using tissues (testis, epididymis) and blood samples of mice. Mice exposed to DEP have a significant increase in body weight, LH level, and seminiferous tubule lumen diameter and decrease in the gonado-somatic index, testosterone level, sperm count, and seminiferous tubule diameter. Additionally, histopathology of testes showed interstitial space dilations, exfoliations, Leydig cell atrophy, germ cell degenerations, and spermatid retention in DEP-exposed testes sections. However, concomitant use of honey and DEP had shown a significant improvement in histopathological lesions, steroid hormone levels, and healthy sperm count. By these results, it is concluded that honey possessed antioxidant potential that can efficiently protect DEP-induced anomalies in male mice.
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http://dx.doi.org/10.1007/s11356-021-14774-wDOI Listing
June 2021

Dietary Black Seed Effects on Growth Performance, Proximate Composition, Antioxidant and Histo-Biochemical Parameters of a Culturable Fish, Rohu ().

Animals (Basel) 2020 Dec 29;11(1). Epub 2020 Dec 29.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.

This feeding trial was conducted to investigate the effects of dietary black seed () supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes levels were decreased for black seed fed rohu. The present study showed that tested black seed levels are safe for rohu. Black seed is cheaply available in local markets of Pakistan; therefore, based on the results of the present study, it is suggested that black seed has potential to be used as natural growth promoter and antioxidant in the diet of rohu.
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http://dx.doi.org/10.3390/ani11010048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824491PMC
December 2020

Abnormal steroidogenesis, oxidative stress, and reprotoxicity following prepubertal exposure to butylparaben in mice and protective effect of Curcuma longa.

Environ Sci Pollut Res Int 2021 Feb 28;28(5):6111-6121. Epub 2020 Sep 28.

Developmental Biology Laboratory, Department of Zoology, University of the Punjab, Lahore, 54000, Pakistan.

Mammalian reproduction is a highly regulated process that can be distorted following exposure to synthetic antimicrobial preservatives like butylparaben (BP). Besides, studies have not investigated the potential antioxidant effects of turmeric on BP-provoked reprotoxicity. The present research was planned on prepubertal mice, orally treated with BP (150 μg/g body weight/day) with and without Curcuma longa (turmeric) (400 μg/mice/day) from postnatal day 35 to 65 routinely. Results showed an insignificant reduction in body weight of both sexes but contrary to these, gonadal weight increased significantly in PB-exposed mice. Additionally, elevated levels of follicle-stimulating hormone and luteinizing hormone while decreased estrogen levels were observed in BP-treated females against control. Sperm count and motility were disturbed, coupled with abnormal sperm morphology in BP-intoxicated group. These findings were synchronized with a decreased testosterone levels in the same group as compared with control. The follicular count revealed reduction in the number of antral follicles while an increase in empty follicles. The BP also significantly increased lipid peroxidation and decreased glutathione content, superoxide dismutase, and catalase activities, while the morphometric, biochemical, and histological deviations were less pronounced in the group, which was co-administered with BP and turmeric. Results indicated that turmeric has antioxidant potential to protect BP-induced oxidative stress and reprotoxicity in mice.
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http://dx.doi.org/10.1007/s11356-020-10819-8DOI Listing
February 2021

Butylated hydroxytoluene protects bull sperm surface protein-P25b in different extenders following cryopreservation.

Vet World 2019 Apr 11;13(4):649-654. Epub 2020 Apr 11.

Biotechnology Research Programme, National Animal Production Research Institute, Zaria, Nigeria.

Aim: The aim of this study was to investigate the effects of different concentration of butylated hydroxytoluene (BHT) on sperm membrane surface protein "P25b" from cryopreserved bull semen in either lecithin based Bioxcell (BX) or two egg-yolk based extenders, tris-egg yolk (TEY), and citrate-egg yolk (CEY).

Materials And Methods: Forty-five semen samples, 15 each were extended with either BX, TEY, or CEY extender which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0, and 3.0 mM/mL) of BHT. The extended semen samples were frozen at a concentration of 20×10/mL in 0.25 mL straws and stored in liquid nitrogen for 2weeks. The frozen samples were thereafter thawed, proteins extracted and analyzed for quantities of protein P25b through direct sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel densitometry. Peptides were confirmed by Western blotting (WB).

Results: Results showed that supplementation of BHT improved (p<0.05) quantity of protein P25b at concentrations of 0.5mM/mL for BX and at 1.0 mM/mL for TEY and CE when compared with the controls and other treatments.

Conclusion: BHT supplementation at 0.5 in BX and 1.0 mM/mL in TEY and CEY has protected bull sperm fertility marker protein P25b in frozen-thawed bull sperm.
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http://dx.doi.org/10.14202/vetworld.2020.649-654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245718PMC
April 2019

Healing potential of Adiantum capillus-veneris L. plant extract on bisphenol A-induced hepatic toxicity in male albino rats.

Environ Sci Pollut Res Int 2018 Apr 14;25(12):11884-11892. Epub 2018 Feb 14.

University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore, Pakistan.

Bisphenol A (BPA) is a widely used environmental pollutant in the production of plastics but causes hepatotoxicity in mammals. In the present study, we studied the BPA-induced oxidative stress in rats and ameliorative potential of Adiantum capillus-veneris L. plant. It was concluded that the BPA can reduce the body and liver weight, increase in biochemical levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), total bilirubin, and disturb the normal hepatic physiology, histology, and metabolism. Additionally, liver histology shows hepatic necrosis, congestion, and vacuolization in exposed individuals. In contrast, simultaneous exposure of A. capillus-veneris and BPA showed declining trend in serum biomarker levels and normal histopathological structures. We conclude that the A. capillus-veneris plant is antioxidant in nature and can reduce the BPA-induced toxicity. These findings are very helpful to understand the BPA-induced hepatic toxicity and ameliorative potential of A. capillus-veneris plant and are of great importance in risk assessment of xenobiotics.
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http://dx.doi.org/10.1007/s11356-018-1211-3DOI Listing
April 2018

Surgical Site Infection Following Fixation of Acetabular Fractures.

Hip Pelvis 2017 Sep 6;29(3):176-181. Epub 2017 Sep 6.

Department of Orthopaedic Surgery, Liaquat National Hospital, Karachi, Pakistan.

Purpose: Acetabular fractures are mainly caused by high energy trauma. Surgical fixation of these fractures requires extensive surgical exposure which increases the length of operation and blood loss as well. This may increase the risk of surgical site infection. Our aim is to evaluate the prevalence of surgical site infections and the risk factors associated with it so as to minimize its chances.

Materials And Methods: A total of 261 patients who underwent acetabular fracture surgery were retrospectively reviewed. Patients were divided into 2 groups, with or without surgical site infection. Factors examined include patients' gender, age, body mass index (BMI), time between injury and surgery, operative time, estimated blood loss, number of packed red blood cell transfused, length of total intensive care unit (ICU) stay, fracture type, surgical approach, smoking status, patients' comorbids and associated injuries.

Results: Fourteen patients (5.4%) developed surgical site infection. Out of 14 infections, 4 were superficial and 10 were deep. The factors that were found to be associated with surgical site infection following acetabular fracture fixation were prolonged operation time, increased BMI, prolonged ICU stay, larger amount of packed red blood cell transfused and associated genitourinary and abdominal trauma.

Conclusion: In our study, we conclude that measures should be undertaken to attenuate the chances of surgical site infection in this major surgery by considering the risk factors significantly associated with it.
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http://dx.doi.org/10.5371/hp.2017.29.3.176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5612977PMC
September 2017

Bisphenol A exposure and healing effects of Adiantum capillus-veneris L. plant extract (APE) in bisphenol A-induced reproductive toxicity in albino rats.

Environ Sci Pollut Res Int 2016 Jun 3;23(12):11645-57. Epub 2016 Mar 3.

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.

The current study presents the bisphenol A exposure and the ameliorative effects of Adiantum capillus-veneris on testicular toxicity induced by bisphenol A. Adult male albino rats were divided into five groups of five animals each: A (control), B (vehicle control), C (toxic), D (protective), and E (ameliorative) were served distilled water, olive oil, bisphenol A (BPA) at 100 mg/kg body weight, A. capillus-veneris plant extract at 25 mg/kg body weight, and BPA + A. capillus-veneris, respectively. All of the doses were administered orally for 15 days, and the rats were then sacrificed. Blood samples for the testosterone assay and both testes were collected for histological examination. The body weight, paired testes weight, relative tissue weight index, Johnsen scoring of tubules, and level of serum testosterone decreased in BPA-treated rats. Similarly, histological examination of the testes in BPA-treated animals revealed a lower number of Leydig cells, an irregular basement membrane, sloughing of germinal layers, vacuolization, a lower number of spermatocytes, and debris in the lumen. However, co-administration of A. capillus-veneris with BPA increased the total antioxidative capacity (330.82 ± 22.46 μmol/mg protein) of the testes and restored the serum testosterone level (1.70 ng/ml); histological features showed restoration in the stages of spermatogenesis. Conclusively, A. capillus-veneris plant extract overcomes the estrogenic effects of BPA on the reproductive system of rats and protects rats' testes against BPA-induced injury/damage via an antioxidative mechanism that appears to be conciliated.
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http://dx.doi.org/10.1007/s11356-016-6330-0DOI Listing
June 2016

First proteomic assay of Pakistani Pisum sativum L. germplasm relation to geographic pattern.

Genetika 2009 Jul;45(7):920-5

Department of Botany, University of Malakand, NWFP, Pakistan.

Proteomic assay was carried out to asses genetic diversity in relation to geographic pattern in 97 genotypes of Pisum sativum L., collected from all over Pakistan. In total 34 bands were observed and among these, 26.7% bands were monomorphic, while 73.5% bands showed polymorphism. Based on both Province Wise Analysis (PWA) and Agro-ecological Zones (AEZ) the genotypes collected from Punjab, North West Frontier Province (NWFP) exhibited 70.6%, and 64.7% variation respectively. The germplasm collected from Azad Kashmir showed the lowest level of genetic diversity. Cluster analysis exhibited, moderate level of association, between genetic diversity and geographic pattern of the genotypes.
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July 2009

Rhabdochona kharani sp. n. (Nematoda: Rhabdochonidae) from the fish Labeo gedrosicus Zugmayer, 1912 from Garruk, District Kharan, Balochistan, Pakistan.

Turkiye Parazitol Derg 2006 ;30(1):62-7

Department of Zoology University of Balochistan, Quetta, Pakistan.

A new nematode species, Rhabdochona kharani sp. nov., has been isolated from the intestine, stomach and swim bladder of the fish Labeo gedrosicus Zugmayer, 1912 (Fam. Cyprinidae: Cypriniformes) from spring water at Garruk, District Kharan, Balochistan. The new species is characterized largely by the presence of eight anterior teeth in the prostom, inconspicuous deirids, by the shape and length of unequal spicules (0.08 - 0.09 and 2.9 - 3.1 mm), and by 17-18 pairs of caudal papillae including 6-7 postanal, one adanal, and 10 -11 preanal. Other characteristics include a pointed tail tip, non filamented eggs, a bulbous, voluminous, vaginal complex that is directed backward, and a vulva that is post-equatorial with a triangular large anterior lip and a smaller posterior lip.
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August 2007

Embryotoxic and teratogenic effects of hexavalent chromium in developing chicks of Gallus domesticus.

Bull Environ Contam Toxicol 1998 Sep;61(3):281-8

Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.

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http://dx.doi.org/10.1007/s001289900760DOI Listing
September 1998

Hexavalent chromium-induced congenital abnormalities in chick embryos.

J Appl Toxicol 1998 May-Jun;18(3):167-71

Department of Zoology, University of the Punjab, Lahore, Pakistan.

The embryotoxic and teratogenic potential of hexavalent chromium was evaluated in chick embryos. Different concentrations of aqueous solution of potassium dichromate ranging from 1.00 to 100.00 microg per egg were injected into chick eggs before incubation. A dose-dependent embryonic mortality was observed in all the groups examined on days 7 and 14 of incubation. The following malformations were observed in survivors: reduced body size and organs; microphthalmia; micromelia; everted viscera; abnormal and twisted neck, beak and spinal cord; isolated epicarditis; club foot; haemorrhage; and patchy feathers. Total mortality showing embryonic resorption was noted in higher dose groups (25-100 microg per egg) on day 14 of incubation.
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http://dx.doi.org/10.1002/(sici)1099-1263(199805/06)18:3<167::aid-jat492>3.0.co;2-8DOI Listing
September 1998